《植物生理学报》 2009, 45(9): 855-858

黄山栾树体细胞胚的发生和组织学观察

冯大领^1,*, 李伟², 张洁¹, 李鹏涛¹, 李明¹, 赵书岗¹, 史宝胜³, 彭伟秀³

1 河北农业大学生命科学学院, 河北保定071001; 2 北京林业大学生物科学与技术学院, 北京100083; 3 河北农业大学园林与旅游学院, 河北保定071001

通信作者：冯大领；E-mail: bjdalingfeng@163.com；Tel: 0312-528241

摘　要：

黄山栾树无菌苗的节间和叶柄离体培养后, 其体细胞胚发生的结果表明: 节间愈伤组织可诱导产生体细胞胚, 而叶柄愈伤组织则生根; 节间愈伤组织诱导培养基为MS+3.0 mg·L-1 2,4-D+0.5~3.0 mg·L-1 NAA; 节间胚性愈伤组织诱导培养基为MS+2.0 mg·L-1 2,4-D; 胚性愈伤组织转移到无植物生长调节剂的MS 培养基上可发育成正常植株。组织学观察表明, 体细胞胚在胚性愈伤组织中有的发生于愈伤组织表层细胞, 有的发生在愈伤组织内部。黄山栾树体细胞胚的形成经历球形胚、心形胚、鱼雷胚和子叶胚几个阶段, 这与合子胚的发育途径相似。

关键词：黄山栾树; 体细胞胚; 胚性愈伤组织; 组织学

收稿：2009-05-25   修定：2009-09-03

资助：北京林业大学林木、花卉遗传育种教育部重点实验室开放基金(05-04)。

Observation of Somatic Embryogenesis and Histology in Koelreuteria bipinnata Franch var. integrifoliola T. Chen

FENG Da-Ling^1,*, LI Wei², ZHANG Jie¹, LI Peng-Tao¹, LI Ming¹, ZHAO Shu-Gang¹, SHI Bao-Sheng³, PENG Wei-Xiu³

¹College of Life Science, Agricultural University of Hebei, Baoding, Hebei 071001, China; ²College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; ³College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding, Hebei 071001, China

Corresponding author: FENG Da-Ling; E-mail: bjdalingfeng@163.com; Tel: 0312-528241

Abstract:

Somatic embryogenesis of Koelreuteria bipinnata var. integrifoliola was studied in this paper. The results showed that somatic embryos were obtained from intersegment callus, and leafstalk callus produced root. Intersegment callus was MS medium+3.0 mg·L^-1 2,4-D+0.5–3.0 mg·L^-1 NAA. Embryonic callus was MS medium+2.0 mg·L^-1 2,4-D. Embryonic callus cells developed to normal seedlings when subcultured on MS medium without plant growth regulator. Histological observation revealed that the somatic embryos produced from cells of superficial callus or cells of inner callus and it successively developed through globular-shaped, heart-shaped, torpedo stages and cotyledon stages, similar to the development of zygotic embryo.

Key words: Koelreuteria bipinnata var. integrifoliola; somatic embryo; embryonic callus; histology